美國Mineralocorticoid(f-hMR) 人鹽皮質(zhì)**受體可誘導表達細胞株
美國PrimCells誘導性穩定細胞株： PrimCells建立的獨有的誘導性細胞高效開(kāi)發(fā)系統，能在極短時(shí)間內篩選出低泄漏，高誘導性的穩定細胞株，成功開(kāi)發(fā)出一系列核受體誘導表達的穩定細胞株產(chǎn)品，為多家知名制藥公司研發(fā)部門(mén)和科研院所提供產(chǎn)品與服務(wù)，廣受用戶(hù)好評。針對客戶(hù)的特定需求，PrimCells亦提供量身定制服務(wù)，通過(guò)密切的客戶(hù)交流和高效的篩選體系，嚴格保證客戶(hù)在3-4周內即獲得高質(zhì)量的誘導性穩定細胞株，具有市場(chǎng)其他供應商無(wú)可比擬的時(shí)效性。
Introduction:
Nuclear hormone receptors (NHRs) are a superfamily of transcription factors that function as powerful metabolic regulators to control a variety of systemic processes in physiology. They also play key roles in the pathophysiology of many major disease states, such as diabetes, obesity, inflammation, atherosclerosis and heart failure. To date, this superfamily has provided a rich source of drug design targets and it is continuing to be one of the hottest areas for pharmaceutical research. PrimCells now provides a comprehensive set of NHR expressing cell lines to the research community. These high-quality, flag- and/or HA-tagged NHR-expressing cell lines aim to facilitate further biochemical and molecular studies of their functions and hence encourage new strategies for drug design.
f-hMR/HEK293 cell line was created by stable co-transfection of HEK293 cells with two plasmids expressing the bacterial Tet repressor (TetR) and the human mineralocorticoid receptor (hMR) proteins. Since the hMR gene is driven by a promoter containing two tetracycline operator (TeTO2) sites, the expression of hMR protein can be induced by doxycycline.
Figure 1 | Confirmed hMR Expression in f-hMR stable cells
美國PrimCells品牌介紹
PrimCells是位于美國加州圣迭戈的一家專(zhuān)業(yè)致力于細胞產(chǎn)品研發(fā)和解決方案提供的業(yè)內技術(shù)領(lǐng)導企業(yè)。
公司匯集了**的生物醫藥技術(shù)專(zhuān)家，獨立開(kāi)發(fā)了多項原代細胞和干細胞相關(guān)的專(zhuān)有技術(shù)。 公司以“細胞研究的選擇”為宗旨，自主研發(fā)超過(guò)70種**產(chǎn)品，填補了多個(gè)特種細胞市場(chǎng)的空白。例如其誘導性表達細胞庫和高效3D皮膚培養系統，皆為該類(lèi)產(chǎn)品目前市場(chǎng)上少數的規?；?。此外，公司以高質(zhì)量的數據與快速穩定的項目完成時(shí)間，長(cháng)期為多家知名制藥公司提供解決方案，獲得用戶(hù)廣泛贊譽(yù)。
PrimCells產(chǎn)品線(xiàn)：
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報告基因干細胞 2. 原代細胞（含報告基因原代細胞） 3. 誘導性穩定細胞株
4. 人源重組蛋白 5. **型培養試劑
PrimCells 解決方案
1.誘導性穩定細胞株開(kāi)發(fā) 2. 原代細胞開(kāi)發(fā) 3. **體外毒理和效能測試 4. 3D-皮膚培養
PrimCells合作伙伴及客戶(hù):
A．生物制藥公司：輝瑞(Pfizer), 諾華(Novartis), 禮來(lái)(Eli2 Lilly),. Amgen, EMD Millipore, Regeneron等公司的毒理和醫藥研發(fā)部門(mén)
B．科研機構：冷泉港實(shí)驗室，哈佛醫學(xué)院, 麻省理工（MIT）,， UCSF, NYU, Salk, UIUC, USC等
